Virus Particles Quantification Service Using TRPS
Virus particles, also known as virions, generally consist of either DNA or RNA molecules surrounded by a capsid comprised of proteins. Since the physical and biological properties of viruses have a significant effect on their specific applications, involving clinical diagnosis, viral therapeutics and so on. Quantitative detection of single virus particles has become a key index and drawn more and more attention. Based on the advancement achieved in electronics technologies as well as nanofabrication, Creative Proteomics is proud to provide a one-stop solution service for our customers using tunable resistive-pulse sensing (TRPS) to perform real-time, sensitive quantification of virus particles. We are committed to promoting our clients' understanding of a variety of viruses in a reliable and effective quantification manner based on this novel nanosensor technology and method.
Tunable Resistive-pulse sensing (TRPS) for high-throughput single particle measurements of virion
TRPS is a variant of resistive pulse sensing (RPS), also a popular experimental technique for high-throughput measurements of individual virus particles. This technique utilizes a stretchable membrane to achieve size-tunable nanopores (the central part of this technique) for counting and measuring particles, and the range of particle size measurements is generally in 60 nm to 2 µm. The advantages of the technique include good accuracy, precision and sensitivity. Previous study had reported that the virus particle measurements by TRPS were in good agreement with electron microscopy (EM) results. Apart from simultaneously determining the size and concentration of virus particles with high resolution, the method can also be used to assess sample stability and to predict attachment behavior of a viral preparation due to its capability of measuring zeta-potential. In addition, TRPS-based measurement requires no pre-staining of samples, therefore a total preparation and measurement time of TRPS for virus particles is relatively short, generally can be achieved in minutes.
Fig1. Schematic section through a tunable pore. (Weatherall, E., & Willmott, G. R. ,2015)
Quantification of virus particles using TRPS
No matter what measurement technique, sample preparation and classification are a critical issue for virus particles quantification. Our staff are professionally trained, enable to provide the most suitable protocols in sample preparation according to our customer's specific research goals. For virion quantification, TRPS has distinct advantages, including efficient size and size distribution measurement, the ability to determine low particle concentrations, as well as charge measurement capability. We have developed TRPS protocols for measuring virus particles in terms of their concentration, size, as well as surface charge. The specific of our services include determination of analysis and measurement protocols, sample preparation, TRPS measurement and analysis as well as data analysis.
The benefits of our service
- Fast and reliable virus quantification
- Determination of viruses with highly resolution, involving virus particle concentration, size, as well as charge
- The most competitive price
- One-stop solution
Creative Proteomics is committed to providing our customers with high quality service and delivering accurate and reliable experimental reports. Here, we are glad to offer virus particle quantification using TRPS system, which has the feature of fast detection, good accuracy, precision and sensitivity. To improve research outcomes and the speed of success, please don't hesitate to contact us for more details.
References
- Weatherall, E., & Willmott, G. R. (2015). "Applications of tunable resistive pulse sensing." Analyst, 140(10), 3318-3334.
- Heider, S., & Metzner, C. (2014). "Quantitative real-time single particle analysis of virions." Virology, 462, 199-206.
* For research use only.
