Viral NMR-Based Metabolomics Service
Metabolomics has been applied to a variety of virus infections by utilizing methodologies, such as nuclear magnetic resonance (NMR), liquid chromatography-mass spectrometry (LC-MS), and gas chromatography-mass spectrometry (GC-MS). Creative Proteomics is an international contract research organization (CRO) providing advanced viral metabolomics analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Based on state-of-the-art equipment and wide-ranging expertise in biotechnological applications, we are proud to offer NMR-based metabolomics to advance virus research.
Analytical techniques for metabolic analysis
The two major analytical avenues for metabolic analysis are NMR and mass spectrometry (MS). Both of these techniques have been applied to determine a large number of small molecules coexisting in complex biological mixtures, involving metabolite identification and quantification. Each technique has its own advantages and disadvantages.
MS-based metabolomics
MS, which measures and separates molecules based on their mass charge ratio (m/z), requires three components: an ion source to generate ions, an analyzer, and a detector. Each component has a different type and is suitable for different experiments. Due to its high throughput, high sensitivity and ability to detect more molecules in complex biological mixtures, MS is widely used in metabolomics analysis, including LC-MS, and GC-MS.
NMR-based metabolomics
In NMR analysis, samples are placed in a magnetic field, and nuclei are altered by a non-zero momentum radio pulse. Due to their high sensitivity, c-NMR, P-NMR and H-NMR are commonly used. The sensitivity of the method can be further improved by sample preparation and magnet techniques, such as the combination of liquid chromatography (LC) as well as the introduction of cryogenically cooled probes. NMR can directly identify and quantitatively analyze a large number of analytes. The main advantages of NMR are simple sample preparation, non-destructive, and reproducibility, while the method is limited to molecules at concentrations above 1 μM. Compared to MS-based techniques, NMR is highly automatable and exceptionally reproducible, which makes high-throughput and large-scale metabolomics studies much more feasible. In addition, due to their inherently non-destructive nature, NMR also supports metabolite imaging as well as metabolic analysis of living samples via magnetic resonance spectroscopy (MRS) and magnetic resonance imaging (MRI).
Service offering in Creative Proteomics
We have developed a NMR-based platform to investigate the metabolomic profile of host cells with various virus infections. Our services include NMR sample preparation, spectra processing as well as targeted and/or untargeted multivariate statistical analysis. With our standardized and fully automated platform, the highest quality NMR data and analytical results can be generated.
Benefits of our services
- Minimal sample preparation
- Standardized and fully automated screening
- Highest quality and reproducibility NMR data
- Targeted and/or untargeted multivariate statistical analysis
The workflow of our services
Creative Proteomics is committed to providing high-quality service to greatly accelerate our customers' project progress and success. For more information on how we can help you, please feel free to contact us. We are glad to cooperate with you!
Reference
- Emwas, A. H., et al. (2019). "NMR spectroscopy for metabolomics research." Metabolites, 9(7), 123.
* For research use only.
