Viral Nucleic Acid Preparation Service
Viral metagenomics is a useful approach that can characterize and complete viral diversity isolated from different sources (an organism or an environment) using high-throughput sequencing technologies. The approach can simultaneously detect novel variants of already known viruses and discover completely new viruses, thereby promising to surveil the involvement diseases. For viral metagenomics, viral nucleic acid extraction and amplification is an important part that greatly influences the downstream analysis of sequencing data. Creative Proteomics offers a sensitive and robust workflow of viral nucleic acids extraction and amplification for high-throughput metagenomic sequencing, with the goal to improve the speed and success of worldwide customers’ viral research.
Viral nucleic acid preparation service in Creative Proteomics
As a critical step that can affect the success of downstream applications, we adopt the following optimize protocols to gain more purified nucleic acids, allowing reliable recovery of viral genomes and a good correlation of the relative number of sequencing reads. The protocol includes nuclease digestion and random amplification of RNA and DNA in separate reactions.
Nuclease treatment
Before nucleic acid extraction, the viral samples are treated with DNase and RNase. Nuclease treatment takes advantage of the principle of the presence of a stable virus capsid that protects the viral genome from digestion, thereby only digestion of human DNA containing a lot of repetitive sequences, further enriching the virus reads.
Nucleic acid extraction
Quality of the extracted nucleic acid is important, as impurities or inhibitors in the purified sample can have significant effects on metagenomics samples. Based on viral total nucleic acids purification kit, viral RNA mini kit and automated instruments, we can extract vDNA and vRNA automatically several samples at the same time.
Unbiased nucleic acids amplification
We offer optimize unbiased amplification of nucleic acids via adopting the workflow to process RNA and DNA in two separate reactions.
Workflow of viral nucleic acids preparation service
Our advantages
- Professional technical support
- Optimized protocols according to customers
- Fast delivery and competitive price
With the growing application of viral metagenomics, more optimized protocols of viral nucleic acids extraction and amplification are needed to allow more accurate experimental data. As a forward-looking research institute, Creative Proteomics is devoted to solving the problems in customer's research, depending on the national technology platform and an excellent team of experts. If you need assistance in the related research areas, please contact us.
Reference
- Lewandowska, D.W., et al. (2017). "Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples." Microbiome 5, 94.
* For research use only.
