Viral Proteins PTMs Analysis Service
Creative Proteomics is a leading provider in the field of viral proteomics services. Based on our years of experience and advanced technology platforms, we have developed good relationships with pharmaceutical manufacturers and scientific research institutions. Here, we offer viral protein post-translational modification (PTM) analysis services. Post-translational modifications (PTMs) have the ability to regulate protein functions by altering the stability, activity, interactions of proteins and subcellular localization. PTMs of viral proteins are relevant throughout various stages of the viral life cycle, especially their infections and genome replication. Thus, the understanding of viral PTM regulation plays an important role in the exploration of viral infections and finding potential drug targets.
Fig1. The virus-human protein-protein interaction network. (Xiang, Y., et al, 2020)
PTMs play important roles in the regulation of viral proteins
Post-translational modifications (PTMs) refer to the enzyme-catalyzed covalent modifications of proteins during or after protein biosynthesis, adding spatial and temporal regulation to modulate protein functions. As obligate intracellular parasites, viruses' replication is largely dependent on the protein synthesis machinery of host cells. Therefore, it is not surprising that many viral proteins are influenced by PTMs. In the last few years, multiple viral PTMs have been identified. A recent study has collected experimentally verified viral protein PTMs and analyzed viral PTMs data using computational methods. Through the investigation of the PTM sequence motifs, characteristics of PTM protein domains, and the function of target human proteins, the study generated fresh insight into viral infection mechanisms and highlighted the importance of viral PTM site identification.
- Phosphorylation, N-glycosylation, and SUMOylation of viral protein have the consensus motifs with human proteins.
- The function of human proteins targeted by viral PTM proteins is associated with protein localization, targeting, and translation.
- Viral protein PTM sites are more likely to be enriched in protein domains.
Fig2. Viral protein PTM motifs discovered by motif-x. (Xiang, Y., et al, 2020)
Services provided by Creative Proteomics
To discover PTM sites, the major means is selective enrichment (usual accomplishment with antibodies against the PTM or protein) of specific proteins or PTMs and following by modified peptide identification. In addition to the discovery-driven experiments, we also provide targeted MS/MS methods, including parallel reaction monitoring (PRM) and selected reaction monitoring (SRM) for sensitive monitoring of PTMs on proteins. In order to fast and effective identification of viral protein PTM sites, bioinformatics approaches are applied. Our specific services include analysis of PTMs on viral structural proteins, PTMs on nonstructural and accessory proteins, as well as interfering PTM sites of host proteins by viral proteins. Based on the latest technologies, such as innovative labeling techniques, our services enable the systematic identification of conventional and novel PTMs of viral proteins, which will greatly advance the understanding of the detailed molecular mechanisms behind PTMs of viral proteins.
PTMs prediction analysis services
- Phosphorylation site prediction
- Ubiquitination site prediction
- Glycosylation site prediction
- Other post-translational modification prediction analysis
The workflow of our services
In terms of translational applications, PTMs of viral proteins and the interference of PTMs of host proteins by viral proteins may be attractive targets for therapeutic intervention. Besides, in addition to providing new insights into the exploration of novel antivirals, a better understanding of the PTMs of viral proteins will promote the development of more efficient vaccines. Creative Proteomics aims to accelerate the research progress of worldwide customers by offering optimal strategies and comprehensive data analysis. To improve research outcomes and the speed of success, please feel free to contact us or directly send us an inquiry. We look forward to providing services for your next project.
Reference
- Xiang, Y., et al. (2020). "Bioinformatics analysis and collection of protein post-translational modification sites in human viruses." bioRxiv.
* For research use only.
