Viral Label-Free Quantitative Proteomic Service
To investigate the virus-host protein interactions across different stages of viral infection, relative protein quantification can be conducted using label-based or label-free approaches. Compared with label-based proteomics, label-free approaches are less time-consuming and inexpensive. In recent years, due to its broad adaptability (it can be used on virtually any biological specimen) and the development of user-friendly software, label-free methods for quantitation are becoming more and more popular in the proteomics community. So far, label-free quantification methods have been widly applied to investigate the mechanisms of action of novel antiviral inhibitors, involving SARS-CoV-2, MERS-CoV, HCV, and other RNA viruses.
Overview of label-free quantitative proteomic analysis
The label-free quantitative proteomic analysis are fast, sensitive, and available for large-scale proteome analysis, providing a powerful tool to profile global protein expression. It has been reported that this method performs much better than label-based proteomics in terms of proteome coverage, as there are threefold more proteins that can be repeatedly identified in repeated measurements. In the label-free quantitative proteomic analysis, proteins are digested into a peptide mixture using proteases, then the samples are analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The relative protein abundance is completed by chromatographic peak intensity measurements or spectral counting.
Benefits of label-free quantitative proteomics
Compared with iTRAQ/TMT and other labeling methods, label-free quantitative proteomic analysis has certain advantages, including,
- Cost-effective. No need for expensive isotope labels.
- Simple experimental process.
No need for protein labeling and short time consumption. Additionally, less pre-processing makes more retention of the original information of the sample.
- Broad adaptability.
Suitable for a variety of types of biological specimens and higher coverage of low abundance polypeptides.
- Excellent analytical reproducibility.
The superb run-to-run reproducibility for the label-free method is possible with improved chromatographic retention time calculators as well as highly precise and accurate MS instruments.
Service offering
Based on our well-established proteome technology platforms and experienced scientists, we can provide label-free quantification services with excellent analytical reproducibility. Our services enable the quantitative analysis of various types of samples. We recommend that the client send the original sample unless they are familiar with and have mastered the protein extraction methods used for proteomic analysis.
Workflow of our label-free quantification services
We promise to provide,
- Complete proteomics experiment report, including detailed experimental procedure.
- Comprehensive analysis reports, involving quantitative information statistics, protein quantitative results, and peptide quantitative statistics.
- Personalized bioinformatics analysis service.
- Provide raw data of protein profiling.
Creative Proteomics is an international contract research organization (CRO) providing viral proteomic, metabolomic and other analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our team specializes in quantitative and qualitative proteomics and metabolomics applications based on state-of-the-art MS platforms, coupled with high-performance liquid chromatography technology. If you are interested in our services, please don't hesitate to contact us. We are glad to cooperate with you and to witness your success!
Reference
- Coombs, K. M. (2020). "Update on Proteomic approaches to uncovering virus-induced protein alterations and virus–host protein interactions during the progression of viral infection." Expert Review of Proteomics, 17(7-8), 513-532.
* For research use only.
