Virus Cultivation and Isolation Service
Viruses live on the host for survival, which are obligatory intracellular parasites. Being different with bacteria, many of which can be grown on non-living culture media or on agar plates alone, viruses require a living host cell to support their replication. Creative Proteomics is a forward-looking research institute as well as a leading provider in the field of virus research. We offer a one-solution service for viral sample collection and processing, virus cultivation and identification. Our customized and flexible service allows for the generation of virus cultures that can be used for downstream analyses and multiple research goals, including cultivation and isolation viruses in clinical samples, virus related research (eg., viral structure, and effects on host cell), and preparation of viruses for vaccine production.
The method of viral isolation and cultivation
Animal inoculation
The natural host is the ideal animal host for study of viruses, and animal inoculation is a platform, which provides specific pathogen free animal for viral cultivation. The method allows for determination of diagnosis, pathogenesis and clinical symptoms. Besides, the production of antibodies can also be identified. Therefore, the method can be used for the application of studying immune responses, epidemiology and oncogenesis. However, the method has the disadvantage of expensive, difficulties in maintenance of animals as well as animal welfare issues.
Egg-based production system
Embryonated egg inoculation is a widely used method of viral isolation and growth, which is more economical and convenient than animal inoculation. The method provides ideal substrate for viral growth and replication, allowing many viruses cultivation in various parts of the egg. Moreover, it is used for rapid isolation of a few viruses, as well as vaccine or antigen production.
Fig1. Embryonated egg inoculation. (Eisfeld, A. J., et al. 2014)
Cell culture technique
Cell culture technique serves as a convenient and cost-effective approach for the isolation, identification and cultivation of viruses. Compared to the above method, the cell-based production systems enable more reliable and well-characterized viruses as well as faster and shorter production cycles due to its easy process control. According to different types of cells, the cell-based production systems can be divided into three categories, including primary cell culture, semi-continuous cell lines, and continuous cell lines.
Virus isolation and cultivation service in Creative Proteomics
Virus isolation and cultivation is a basic, reliable and widely used method for establishing a diagnosis of viral infection. Although the advent of novel methods, such as RT-PCR, has fundamentally changed the isolation of virus in the lab setting, virus isolation and cultivation remain a standard method for the direct diagnosis of many viral infections. The method is very specific for viral infection, but requires a long time for completion, specialized laboratory equipment as well as skills. To support the viral research of our customers, we provide virus isolation and cultivation service according to the specific projects, involving experimental design, sample collection and treatment, and virus cultivation and identification. Our services are customized and flexible, which can be regulated to the specific research objectives and experimental budget.
The application of our service
- Virus detection/identification
- Cultivate and isolate viruses in clinical samples
- Virus related research (such as, viral structure, replication and effects on host cell)
- Vaccine production
Creative Proteomics is your professional and reliable partner in the field of viral research. With a focus on professional and first-class services as well as the exact needs of our customers, we are committed to providing the finest service experience. If you are interested in our services, please contact us for more details. We are looking forward to cooperating with you!
References
- Eisfeld, A. J., et al. (2014). "Influenza A virus isolation, culture and identification." Nature protocols, 9(11), 2663.
- Hematian, A., et al. (2016). "Traditional and modern cell culture in virus diagnosis." Osong public health and research perspectives, 7(2), 77-82.
* For research use only.
