Metagenomic Samples Preliminary Treatments Service
The collection, processing of samples is the premise of virus metagenomics sequencing, which affects both quality and accuracy of metagenomics data, playing a vital role in the success of the experiment. Since the relative scarcity of viral genetic material in metagenomic samples and the process of sample treatment is susceptible to contamination. Therefore, careful preliminary work to optimize processing conditions for different sample types is necessary. As a forward-looking research institute as well as a leading service provider in the field of viral metagenomics, Creative Proteomics provides different metagenomic samples preliminary treatments services for the specific needs of our customers, involving samples pre-processing service, host associated bacteriophage isolation service, viral nucleic acid preparation service.
Precautions for preliminary treatments
Recently, with the growing attention of viral metagenomics, standardized protocols of samples preparation are needed to allow more accurate and fast procedure in a broad application of related research. Key objectives of samples preliminary treatments are to collect sufficient virus biomass for sequencing. Besides, contamination of samples should be minimized. Particularly, metagenomics data sets from low-biomass samples are particularly vulnerable to this problem. Previous studies have demonstrated that different collection and storage protocols and conditions can affect the viral community profiles, such as the length of time between sample collection and freezing and the number of freeze-thaw cycles a sample undergoes. In addition, DNA/RNA extraction method has a great influence on the composition of downstream sequence data. Notably, the extraction method should be effective for diverse viral taxa.
Browse the following available services that we provide:
Creative Proteomics offers a series of metagenomic samples preliminary treatments services which are part of our integrated portfolio of virus detection and discovery service. With experienced teams of technicians under unified and standard operation. We are committed to satisfying worldwide customers with first-class services at competitive prices. If you are interested in our services, please contact us for more details.
Reference
- Quince, C. et al. (2017). “Shotgun metagenomics, from sampling to analysis.” Nature biotechnology, 35(9), 833-844.
* For research use only.
