Real-Time PCR for Virus Detection

Conventional methods for virus detection are not only labourious but also time consuming. Therefore, a rapid and accurate laboratory detection method of virus has emerged. Real-time PCR (also denoted as quantitative PCR—qPCR), an innovation of PCR, represents a powerful tool in microbial diagnostics. The method is characteristics by extremely sensitive and accurate, and its suitability of virus diagnostics is beyond doubt. Since the application of 2009 fluA/H1N1 pandemic, real-time PCR has been widely used for virus detection, involving viral detection, quantification and typing. As a forward-looking research institute as well as a leading service provider in the field of viral detection, Creative Proteomics provides virus detection service using real-time PCR to meet the special need of world customers.

An important technique for the detection and monitoring of virus

Real-time PCR is the gold standard for fast, sensitive and precise detection of target nucleic acids. The method is based on the continuous measurement of the variation of fluorescence signals during the amplification reaction, which permits the detection of the number of amplicons generated during each amplification cycle in a real-time mode. Due to appropriate calibration and the use of standard curves, real-time PCR allows the assessment of absolute copy numbers of the target. Owing to the unique features, real-time PCR has become one of the most important techniques for the detection and monitoring of viruses. The method for virus detection can be performed in multiple types of samples, and is widely used in the application of assessment of viral load, determination of virus proliferation, and among others.

Virus detection service in Creative Proteomics using real-time PCR

In Creative Proteomics, we provide a one-stop service of qPCR-based virus detection, including consultation and design of experimental ideas, DNA/RNA extraction from multiple types of samples, primer design and validation, design of probes, data analysis and so on. With our team of well-trained experts, state-of-the-art instruments and robust workflows, we are committed to providing our customers with the most reliable and efficient research services at a competitive price. The specific workflow is below.

Viral Detection tailored for research applications

• Animal health
• Food testing
• Environmental testing

Simply let us know about your research needs. We will propose the best strategy to best match your research objectives. Contact us right now!

Reference

1. Watzinger, F., et al. (2006). "Detection and monitoring of virus infections by real-time PCR." Molecular aspects of medicine, 27(2-3), 254-298.

* For research use only.