Acetylation is another common post-translational modification (PTM) of proteins and plays an important role in many cellular physiological and pathological processes. Protein acetylation modifications are beginning to be in full swing in the cell cycle, DNA damage research, apoptosis, autophagy, metabolism, immunity, and other fields. Creative Proteomics is a leading custom service provider with extensive experience in the analysis of protein PTMs. Here, we are proud to offer an acetylated peptide enrichment service. We are committed to greatly improving our customers' understanding of acetylation and helping them further investigate the function of lysine acetylation in eukaryotes and prokaryotes.
Protein acetylation
Protein acetylation is the process by which the acetyl group on acetyl coenzyme A (Ac-CoA) is transferred to a specific position on a polypeptide chain and is one of the major PTMs in eukaryotes. Protein acetylation was first found to occur mainly on histones in the nucleus and is involved in the regulation of gene transcription processes. As the sensitivity of protein detection by MS increases, more and more non-histone proteins are found to undergo acetylation modifications as well. Acetylation interferes with metabolic processes and energy homeostasis, not only due to the consumption of Ac-CoA during acetylation but also due to the consumption of NAD+ during deacetylation. It has been reported that malfunctioning acetylation mechanisms can lead to serious diseases such as metabolic disease, neurodegenerative diseases, cardiovascular diseases, and cancers.
Acetylated peptide enrichment service
Due to the nature of proteomics approaches involving the acquisition of large amounts of information from a single sample, acetylome studies often require careful optimization to reduce background, with enrichment of acetylated peptides from complex mixtures being a key step. Creative Proteomics has developed a systematic acetylated peptide enrichment workflow, including peptide prefractionation and enrichment of acetylated peptides of complex mixtures.
- Acetylated peptide prefractionation
-Strong cation exchange chromatography (SCX).
-Hydrophilic interaction chromatography (HILIC).
-Reverse phase liquid chromatography (RPLC). - Acetylated peptide enrichment—pan acetylation antibody-based affinity enrichment
In mass spectrometry (MS) studies of acetylome, in order to increase the number of true positives, it is necessary to first enrich for acetylated lysine peptides to remove the non-acetylated peptides. Pan-acetyl lysine antibody-based affinity enrichment is the most commonly used method for the enrichment of acetylated peptides. Prior to immunoprecipitation with pan-acetyl lysine antibodies, we typically use trypsin to cleave large proteins into multiple small peptides as well as expose the acetyl lysine to enhance affinity enrichment. It is worth noting that in order to improve the enrichment efficiency of acetylated peptides, we often combine the above fractionation method with the acetylation enrichment method.
Sample requirements
- Acceptable samples: Protein extracts, cell samples, tissue samples, body fluid samples (such as blood and urine), and microorganism samples.
- Sample shipping: Sufficient amount of dry ice for shipping, or consult our technical staff before sending samples.
- Before the formal experiment, we will always test the samples you provide.
Our services are committed to helping our customers to get insight into the biological and pathological roles of protein acetylation and acetylated proteins. If you are interested in our acetylated peptide enrichment service, please feel free to contact us for information.
Reference
- Diallo, Issa, et al. "Current trends in protein acetylation analysis." Expert Review of Proteomics 16.2 (2019): 139-159.
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