PTM Solutions — Drug Discovery, Biopharma Characterization & Basic Research

Comprehensive PTM Solutions for Research and Development

Post-translational modifications regulate virtually every aspect of protein function — from enzyme activity and protein-protein interactions to protein stability and cellular localization. Understanding PTMs is essential for drug target validation, therapeutic protein development, and fundamental biological research.

Our PTM solutions are organized into three application areas. Drug Discovery Solutions cover the preclinical pipeline including PTM enzyme activity assays, inhibitor screening, protein degrader efficacy evaluation, and covalent drug profiling. Biopharma Solutions provide comprehensive PTM characterization for therapeutic proteins, with a focus on glycosylation analysis across all major expression systems. Research Solutions extend our PTM platforms to model organisms and disease contexts.

Drug Discovery Solutions — Target Identification to Candidate Selection

Our drug discovery PTM portfolio covers the full pipeline from target identification and enzyme activity assays to degrader efficacy evaluation and covalent drug profiling.

PTMs in Drug Discovery — A comprehensive overview of how post-translational modifications impact drug development across therapeutic areas. View service →

Enzyme Activity & Target Discovery

• DUB and Ubiquitin Enzyme Activity Assays — Deubiquitinase and E3 ligase activity measurements using fluorogenic substrates and ubiquitin chain assembly assays.
• HDAC / HAT Activity Assay Services — HDAC1-11, SIRT1-7 and HAT activity profiling using fluorometric and LC-MS assays for epigenetic drug screening.
• Kinase Activity Profiling Service — Kinase activity measurement across 400+ kinase targets using radiometric and label-free MS assays.
• PTM Enzyme Activity & Inhibitor Screening — Integrated platform combining PTM enzyme assays with high-throughput inhibitor screening.

Protein Degrader Solutions — Targeted protein degradation requires specialized proteomics approaches. Our platform integrates seven complementary strategies:

Covalent Drug & Cysteine Profiling

• Covalent Drug PTM Profiling — Proteome-wide identification of covalent drug-modified cysteines using isotopic tandem labeling and LC-MS/MS. Supports KRAS G12C, BTK, and EGFR-targeting compounds.
• Reactive Cysteine Target Engagement Assay — Quantitative cysteine reactivity profiling using competitive ABPP for selectivity ranking and off-target identification.

Epigenetic Research

• Epigenetic PTM Research Services — Histone modification analysis, chromatin PTM profiling, and writer/eraser/reader characterization for epigenetic drug discovery.

Biopharma Solutions — PTM Characterization for Therapeutic Proteins

Post-translational modifications critically impact the safety, efficacy, and stability of protein therapeutics. Our biopharma PTM characterization platform provides comprehensive analysis across all major modification types and expression systems.

• Biopharma PTM Characterization — Comprehensive PTM mapping for monoclonal antibodies, fusion proteins, and bispecifics. Covers deamidation, oxidation, isomerization, and glycation for comparability studies.
• ADC / Fusion Protein PTM Analysis — Drug-to-antibody ratio determination, conjugation site mapping, and linker stability assessment for ADCs and fusion proteins.

Glycosylation Analysis

• Antibody Glycosylation — N-glycan and O-glycan profiling for monoclonal antibodies. Fc glycan heterogeneity, galactosylation, fucosylation, and sialylation analysis.
• Protein Drug Glycosylation — Comprehensive glycosylation characterization for non-antibody therapeutic proteins including fusion proteins, cytokines, and enzymes.
• Protein PEGylation — PEG attachment site mapping and PEGylation degree quantification using specialized LC-MS/MS approaches.

Expression System-Specific Glycosylation

• Bacterial Glycosylation — N-linked and O-linked glycosylation in engineered glycosylation-competent bacterial strains.
• Insect Cell Glycosylation — Glycan profiling for Sf9, Sf21, and High Five insect cell expression systems.
• Yeast Glycosylation — High-mannose and engineered human-like glycosylation analysis for P. pastoris and S. cerevisiae.
• Protein Drug PTM Mapping — Site-level PTM identification under stress conditions for forced degradation and formulation stability assessment.

Research Solutions — PTM in Model Organisms and Disease

For academic and fundamental research programs, our PTM analysis platforms extend across diverse biological systems and disease contexts.

• Bacterial PTM Research — Bacterial PTM profiling including acetylation, phosphorylation, and novel modifications in pathogenic and non-pathogenic strains.
• Biological Research — Broad PTM discovery for signaling pathways, cell cycle regulation, and stress response mechanisms.
• Disease Research — PTM profiling in cancer, neurodegenerative disorders, metabolic disease, and inflammation for biomarker discovery.
• Plant PTM Research — PTM analysis in plant model organisms and crop species covering phosphorylation, acetylation, and succinylation.
• Viral PTM Research — PTM profiling of viral proteins and infected host cells for antiviral target identification and replication mechanism studies.

Frequently Asked Questions About PTM Solutions

References

1. Cohen P. The role of protein phosphorylation in human health and disease. European Journal of Biochemistry. 2001;268(19):5001-5010. doi:10.1046/j.1432-1327.2001.02473.x
2. Walsh G, Jefferis R. Post-translational modifications in the context of therapeutic proteins. Nature Biotechnology. 2006;24(10):1241-1252. doi:10.1038/nbt1252
3. Bond MJ, Crews CM. Proteolysis targeting chimeras (PROTACs) come of age. RSC Chemical Biology. 2021;2(3):725-742. doi:10.1039/D1CB00011J
4. Apweiler R, Hermjakob H, Sharon N. On the frequency of protein glycosylation. Biochimica et Biophysica Acta. 1999;1473(1):4-8. doi:10.1016/S0304-4165(99)00165-8

*This service is provided for research use only (RUO). It is not intended for clinical diagnostic or therapeutic applications.