Peptide Enrichment for Mass Spectrometry (MS)-Based Analysis

To overcome these issues of PTMs, enrichment strategies of the modified peptides of interest have been developed prior to MS analysis, such as ion exchange chromatography (IEC), immunoaffinity precipitation, and immobilized metal affinity chromatography (IMAC).

MS-Based Post-Translational Modification Analysis

MS is currently the gold standard and the most informative technique for protein analysis. Similar to conventional protein identification, MS-based methods for PTM analysis include top-down, bottom-up, and middle-down proteomics.

Protein Post-Translational Modification Quantitative Analysis

Beyond identification, we also apply MS-based approaches for the quantification of PTMs. We specialize in a wide range of PTM analyses, including phosphorylation, acetylation, glycosylation, and ubiquitination, among others.

Protein Post-Translational Modification Bioinformatics Analysis

Based on our experienced experts and popular tools , we are able to provide PTM site prediction services, such as phosphorylation site, glycosylation site, acetylation site, methylation site, SUMOylation site and ubiquitination site prediction.

Diverse Peptide Enrichment Strategies

MS-Based Proteomics Methods

Various Labeling and Label-Free Strategies

Advanced Bioinformatics Tools

PTM Proteomics Analysis Platform

By combining modified peptide enrichment strategies and mass spectrometry (MS)-based analysis, Creative Proteomics can achieve a systematic analysis of protein PTM states in protein complexes or cellular structures and explore PTM crosstalk that form complex assembly and composition. In addition to MS-based proteomics analysis, protein microarray is another powerful technique for screening PTMs, improving our capability of identifying PTM events in an easier and unbiased manner.

High-Precision, High-Resolution Mass Spectrometry (MS)

Based on advanced mass spectrometers, we offer different MS-based strategies, including bottom-up, middle-down, and top-down proteomics. To achieve a comprehensive protein PTM analysis, these approaches can be combined. We have experienced MS-based proteomics scientists and technicians and are dedicated to providing our customers with quality PTM characterization services.

High-Quality Antibody-Based Protein Microarray

We have established a high-quality antibody-based protein microarray platform for the global profiling of a wide range of protein PTMs. Reverse phase protein microarrays (RPPAs) are characterized by high multiplex and the ability to simultaneously analyze thousands of proteins and their PTMs that may be present in the crude cells or tissue lysates.

In the last several decades, a large amount of protein sequence data has been generated, highlighting the importance of the in-depth study and analysis of protein PTMs. Creative Proteomics strives to be a guide and assistant in protein PTM research for our customers worldwide. Our customer service representatives are available 24 hours a day from Monday to Sunday. Contact us now for all the details!